When these polymers are used for modification of enzymes, less binding affinity is required to introduce more PEG chains onto the enzyme surfaces than that of a single polymeric PEG modifier. Consequently, the residual activities of the modified enzymes would become higher.
General Characteristics for PEG SUNBRIGHT® AM series
Moreover, with the use of this compound, the hydrophilic/lipophilic balance can be adjusted by changing the composition ratios of the ethylene oxide and propylene oxide in the polyalkylene glycol present in the nucleus of this compound.
Efficacy of Comb-type PEG Enzyme Conjugation
Stability of Modified Protease in water (at 40℃)
Stability of modified L-Asparaginase
Residual activity and Antigenicity of Modified L-Asparaginase
References: for modified enzymes using SUNBRIGHT AM-series:
(1) Kajiuchi, J., Park, J. W. (1992) J. Chem. Eng. Japan. 25, 202
(2) Masunaga, T., et al. (1993) Journal of SCCJ. 27, No.3, 276
(3) Sasaki, H., et al. (1993) Biochem. Biophys. Res. Commun. 197, 287
(4) Kodera, Y., et al. (1994) Bioconjugate Chem. 5, 283
(5) Matsuo, K., et al. (1994) Adv. Bioseparation Eng. 1993, 3, 56
[↑]Top
